Development of Rice Introgression Lines with Brown Planthopper Resistance and KDML105 Grain Quality Characteristics through Marker-assisted Selection

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Published: Jun 30, 2009
Keywords:
brown planthopper Nilaparvata lugens marker-assisted selection SSR allele KDML 105

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Jirapong Jairin
Ubon Ratchathani Rice Research Center
Waraporn Wongboon
Ubon Ratchathani Rice Research Center
Kittiphong Phengrat
Surin Rice Research Center
Sa-nguan Teangdeerith
Ubon Ratchathani Rice Research Center
Phikul Leelagud
Ubon Ratchathani Rice Research Center
Kalaya Sansen
Ubon Ratchathani Rice Research Center

Abstract

In this study, a simple sequence repeat (SSR) analysis was performed to identify and localize the Bph3 gene derived from cv. Rathu Heenati. For mapping of the Bph3 locus, the backcross population, BC3F2, from a cross of Rathu Heenati x KDML 105 was developed and evaluated this for BPH resistance. Thirty-six polymorphic SSR markers on chromosomes 4, 6 and 10 were used to survey 15 resistant and 15 susceptible individuals from the backcross population. One SSR marker, RM190, on chromosome 6 was associated with resistance and susceptibility in the backcross population. Additional SSR markers surrounding the RM190 locus were examined to define the location of Bph3. Based on the linkage analysis of 333 BC3F2 individuals, it could be able to map the Bph3 locus between two flanking SSR markers, RM588 and RM589, on the short arm of chromosome 6. The tightly linked SSR markers were further used to develop introgression lines (ILS) with essential grain quality traits and BPH resistance. The linkage drag between Bph3 and Wxa alleles was successfully broken by phenotypic selection integrated with marker-assisted selection. All fifty-one selected ILs developed in this study showed a broad spectrum resistance against BPH populations in Thailand and had KDML 105 grain quality standards. Finally this study was revealed that the ILs can be directly developed into BPH resistance varieties or can be used as genetic resources of BPH resistance to improve rice varieties with the Wxballele in rice breeding programs.

Article Details

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Vol. 3 No. 1 (2552): January - June 2009
Section
Articles

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