Genetic Diversity and Biotypes of the Rice Gall Midge in Thailand
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Abstract
An amplified fragment length polymorphism (AFLP) and biotype-specific markers were used for identification of genetic diversity and biotypes of rice gall midge, Orseolia oryzae in Thailand. Pupae and adults of the gall midges were collected from 19 field locations of 12 provinces during 2006 - 2007. Total of 242 polymorphic AFLP bands were generated from 12 selective primer pairs. Cluster analysis, performed by the UPGMA, separated the gall midge populations into four distinct groups. To identify the biotype, five specific-biotype markers were used to characterize the collected gall midges. Two biotypes of the gall midges, biotype 2 and 5, were identified from all populations used in this research. The results showed that the genetic diversity was not associated with the biotypes of
gall midges. This research may provide basic information to develop an effective strategy for controlling gall midge outbreak in Thailand.
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